BIOS 223: Development and reporting of robust and reproducible LC-MS/MS assays

Course Description

This mini-course offers a series of lectures and hands-on labs to discuss the development and reporting of reproducible quantitative LC-MS/MS molecular assays using a triple quadrupole. We will discuss chromatography and mobile phase selection, mass spectrometry parameters and selection of fragment ions, and the reporting and interpretation of published methods. Additionally we will cover the use of internal standards and sample preparation, and normalization methods for reproducible data analysis. Students will have the opportunity to work with a mass spectrometer and will design a cohesive plan for a targeted assay of an example molecule in their research.